Free Vendor Seminars
Waters
Corp.
Lunch, Monday, October 24, 2011
12:30 pm – 1:30 pm
Automation of Methods Development and
Routine Operation
for Characterization of Proteins
with Multiple Modes of Chromatography
Complete characterization of proteins requires multiple chromatographic modes
that detect different chemical and physical characteristics of the molecules.
Such separations are most conveniently optimized by adjustments in pH and ionic
strength of the mobile phase. We have developed an automated, systematic approach
to screen those parameters for a wide range column of chemistries. The protocol
revolves around the use of Auto-Blend Plus™ Technology that permits programming
the chromatographic method directly in units of pH and salt concentration. By
combining the control interface with column and buffer selection valves, sample
separation options including anion exchange, cation exchange, and size exclusion
at any desired pH and salt concentration can be tested. The same approach can
be adapted for routine analysis of a particular sample in multiple modes. We
will illustrate these approaches for a variety of protein mixtures, including
monoclonal antibodies.
Phenomenex
Inc.
Lunch, Tuesday, October 25, 2011
12:30 pm – 1:30 pm
Wide Pore Core-Shell Columns for Improving Protein and
Peptide Separations
Phenomenex introduces Aeris, the latest innovation in chromatography of Proteins and Peptides. Aeris incorporates award winning core-shell technology to deliver improved performance for protein and peptide separation. The unique 3.6u widepore core-shell particle delivers UHPLC separation of proteins at column backpressures a fraction of sub-2µm columns and is offered in three unique selectivites: XBC18, XB-C8 and C4. An Aeris Peptide XB-C18 column is also introduced to complement the Aeris wide pore offerings. Aeris Peptide is a narrow pore UHPLC column specifically designed for improved separation of peptides and peptide maps. Aeris peptide features 3.6 µm or 1.7µm particle columns based on the HPLC or UHPLC system being used for peptide separations. The Aeris core-shell product line represents next generation solution for bioseparations.
Seminars will be assigned on a first-come, first-serve basis while available.